In JoVE (1)
Articles by Marcella M. Cline in JoVE
Assessing Spatial Memory Impairment in a Mouse Model of Traumatic Brain Injury Using a Radial Water Tread Maze Marcella M. Cline1, Megan A. Ostlie2, Chloe G. Cross3, Gregory G. Garwin2, Satoshi Minoshima2, Donna J. Cross2 1Department of Radiology, University of Washington, 2Department of Radiology, University of Utah, 3Geriatric Research Education and Clinical Center (GRECC), VA Puget Sound Here we present a protocol for a mouse-specific test of cognition that does not require swimming. This test can be used to successfully distinguish controlled cortical impact-induced traumatic brain injury mice from sham controls.
Other articles by Marcella M. Cline on PubMed
Paclitaxel Improves Outcome from Traumatic Brain Injury Brain Research. Aug, 2015 | Pubmed ID: 26086366 Pharmacologic interventions for traumatic brain injury (TBI) hold promise to improve outcome. The purpose of this study was to determine if the microtubule stabilizing therapeutic paclitaxel used for more than 20 years in chemotherapy would improve outcome after TBI. We assessed neurological outcome in mice that received direct application of paclitaxel to brain injury from controlled cortical impact (CCI). Magnetic resonance imaging was used to assess injury-related morphological changes. Catwalk Gait analysis showed significant improvement in the paclitaxel group on a variety of parameters compared to the saline group. MRI analysis revealed that paclitaxel treatment resulted in significantly reduced edema volume at site-of-injury (11.92 ± 3.0 and 8.86 ± 2.2mm(3) for saline vs. paclitaxel respectively, as determined by T2-weighted analysis; p ≤ 0.05), and significantly increased myelin tissue preservation (9.45 ± 0.4 vs. 8.95 ± 0.3, p ≤ 0.05). Our findings indicate that paclitaxel treatment resulted in improvement of neurological outcome and MR imaging biomarkers of injury. These results could have a significant impact on therapeutic developments to treat traumatic brain injury.
Novel Application of a Radial Water Tread Maze Can Distinguish Cognitive Deficits in Mice with Traumatic Brain Injury Brain Research. Feb, 2017 | Pubmed ID: 27923635 The use of forced-swim, rat-validated cognition tests in mouse models of traumatic brain injury (TBI) raises methodological concerns; such models are vulnerable to a number of confounding factors including impaired motor function and stress-induced non-compliance (failure to swim). This study evaluated the ability of a Radial Water Tread (RWT) maze, designed specifically for mice, that requires no swimming to distinguish mice with controlled cortical impact (CCI) induced TBI and Sham controls.