In JoVE (1)
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Articles by Margaret L. Warner in JoVE
Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS Taylor R. Fore1, Audrey A. Ojwang1, Margaret L. Warner1, Xinyun Peng1, Rudolf A. Bohm1,2, William P. Welch1, Lindsey K. Goodnight1, Hong Bao1, Bing Zhang1 1Department of Zoology, University of Oklahoma - Norman, 2Department of Biology, Brandeis University We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.