Articles by Megan Cheng in JoVE
Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum Gustavo Diaz1, Chandler Bridges1, Megan Lucas1, Yong Cheng2, Jeff S. Schorey2, Karen M. Dobos1, Nicole A. Kruh-Garcia1 1Department of Microbiology, Immunology and Pathology, Colorado State University, 2Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame Here, we present a protocol to purify exosomes from both plasma and serum with reduced co-purification of non-exosomal blood proteins. The optimized protocol includes ultrafiltration, protease treatment, and size exclusion chromatography. Enhanced purification of exosomes benefits downstream analyses, including more accurate quantification of vesicles and proteomic characterization.
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues Tsung-Cheng Lin1, Ying-Chih Liao2, Wen-Tsan Chang1,2, Cheng-Han Yang1, Li-Hsin Cheng1, Megan Cheng3, Hung-Chi Cheng1,2 1The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 3 An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization.