In JoVE (1)
Other Publications (1)
Articles by Mo Baikoghli in JoVE
Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods Chun Chieh Chen*1, Marie Stark*1, Mo Baikoghli1, R. Holland Cheng1 1Department of Molecular and Cellular Biology, University of California Davis We have engineered the capsid protein of hepatitis E virus as a theranostic nanoparticle (HEVNP). HEVNP self-assembles into a stable icosahedral cage in mucosal delivery. Here, we describe the modification of HEVNPs for tumor targeting by mutating surface-exposed residues to cysteines, which conjugate synthetic ligands that specifically bind tumor cells.
Other articles by Mo Baikoghli on PubMed
Nanodelivery of a Functional Membrane Receptor to Manipulate Cellular Phenotype Scientific Reports. | Pubmed ID: 29476125 Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging and produce unwanted side effects. Here we develop and validate a nanodelivery approach to transfer in vitro synthesized, functional membrane receptors into the plasma membrane of living cells. Using β-adrenergic receptor (βAR), a prototypical G-protein coupled receptor, as an example, we demonstrated efficient incorporation of a full-length βAR into a variety of mammalian cells, which imparts pharmacologic control over cellular signaling and affects cellular phenotype in an ex-vivo wound-healing model. Our approach for nanodelivery of functional membrane receptors expands the current toolkit for DNA and RNA-free manipulation of cellular function. We expect this approach to be readily applicable to the synthesis and nanodelivery of other types of GPCRs and membrane receptors, opening new doors for therapeutic development at the intersection between synthetic biology and nanomedicine.