In JoVE (1)
Articles by Oumaima Stambouli in JoVE
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques Nico Grüner1, Oumaima Stambouli1, R. Stefan Ross1 1Institute of Virology, National Reference Centre for Hepatitis C, Essen University Hospital, University of Duisburg-Essen The preparing and processing of dried blood spots (DBS) for their final analysis are still poorly standardized for most diagnostic applications. To overcome this shortcoming, a comprehensive step-by-step protocol is suggested and subsequently evaluated with regard to its effectiveness for detecting markers of viral infections.
Other articles by Oumaima Stambouli on PubMed
Detection of Infections with Hepatitis B Virus, Hepatitis C Virus, and Human Immunodeficiency Virus by Analyses of Dried Blood Spots--performance Characteristics of the ARCHITECT System and Two Commercial Assays for Nucleic Acid Amplification Virology Journal. 2013 | Pubmed ID: 23497102 Nowadays, dried blood spots (DBS) are primarily used to obtain diagnostic access to risk collectives such as intravenous drug users, who are prone to infections with hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). Before DBS analyses can be used in this diagnostic context, however, a comprehensive evaluation of its performance characteristics must be conducted. To the best of our knowledge, the current study presents for the first time such essential data for the Abbott ARCHITECT system, which is currently the worldwide leading platform in this field of infection diagnostics.
A Multicentre Sero-behavioural Survey for Hepatitis B and C, HIV and HTLV Among People Who Inject Drugs in Germany Using Respondent Driven Sampling BMC Public Health. 2014 | Pubmed ID: 25124485 People who inject drugs are at high risk for hepatitis B, hepatitis C and HIV. HTLV was reported by neighboring countries to be prevalent in this population, but the situation for Germany is unclear. To generate seroprevalence and related behavioural data and to enhance prevention efforts against these infections for drug users in Germany, a multicentre sero- and behavioural survey was initiated. People who inject drugs are not well reached by services for testing and counselling for blood-borne infections in Germany. An interventional part of the study is intended to prove feasibility and acceptance of testing and counselling in low-threshold drop-in settings.