In JoVE (1)
Other Publications (0)
Articles by Pablo R. Helguera in JoVE
Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures Ardeshir S. Rahman1, Shaudee Parvinjah1, Michael A. Hanna1, Pablo R. Helguera1, Jorge Busciglio1 1Department of Neurobiology and Behavior, University of California, Irvine Here, we describe a method for efficient cryopreservation and thawing of cortical brain tissue blocks to generate highly enriched neuronal cultures. This simple protocol provides flexibility for later generation of neuronal, astrocyte, and neuronal precursor cell cultures.