Paul Wiseman Departments of Chemistry and Physic McGill University Biography Publications Institution JoVE Articles Paul Wiseman has not added a biography. If you are Paul Wiseman and would like to personalize this page please email our Author Liaison for assistance. Publications Spatial Intensity Distribution Analysis Reveals Abnormal Oligomerization of Proteins in Single Cells Biophysical Journal. Aug, 2015 | Pubmed ID: 26287623 Cholesterol Modulates CFTR Confinement in the Plasma Membrane of Primary Epithelial Cells Biophysical Journal. Jul, 2015 | Pubmed ID: 26153705 Image Correlation Spectroscopy: Principles and Applications Cold Spring Harbor Protocols. Apr, 2015 | Pubmed ID: 25834267 Microstructural and Mechanical Characterization of Scarred Vocal Folds Journal of Biomechanics. Feb, 2015 | Pubmed ID: 25648495 Molecular Flow Quantified Beyond the Diffraction Limit by Spatiotemporal Image Correlation of Structured Illumination Microscopy Data Biophysical Journal. Nov, 2014 | Pubmed ID: 25418107 Collapsin Response Mediator Protein 4 Regulates Growth Cone Dynamics Through the Actin and Microtubule Cytoskeleton The Journal of Biological Chemistry. Oct, 2014 | Pubmed ID: 25225289 Gephyrin Clusters Are Absent from Small Diameter Primary Afferent Terminals Despite the Presence of GABA(A) Receptors The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jun, 2014 | Pubmed ID: 24920633 Bone Ablation Without Thermal or Acoustic Mechanical Injury Via a Novel Picosecond Infrared Laser (PIRL) Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery. Mar, 2014 | Pubmed ID: 24376121 Measuring Ligand-receptor Binding Kinetics and Dynamics Using K-space Image Correlation Spectroscopy Methods (San Diego, Calif.). Mar, 2014 | Pubmed ID: 23938869 Bridging the Gap Between Single Molecule and Ensemble Methods for Measuring Lateral Dynamics in the Plasma Membrane PloS One. 2013 | Pubmed ID: 24324577 A Nu-space for ICS: Characterization and Application to Measure Protein Transport in Live Cells New Journal of Physics. Aug, 2013 | Pubmed ID: 24223019 Spatial Intensity Distribution Analysis (SpIDA): a New Tool for Receptor Tyrosine Kinase Activation and Transactivation Quantification Methods in Cell Biology. 2013 | Pubmed ID: 24143969 Fluctuation Imaging Spiced Up with a Piece of PIE Biophysical Journal. Aug, 2013 | Pubmed ID: 23972830 Heat Generation During Ablation of Porcine Skin with Erbium:YAG Laser Vs a Novel Picosecond Infrared Laser JAMA Otolaryngology-- Head & Neck Surgery. Aug, 2013 | Pubmed ID: 23949359 Image Correlation Spectroscopy for Measurements of Particle Densities and Colocalization Current Protocols in Cell Biology / Editorial Board, Juan S. Bonifacino ... [et Al.]. Jun, 2013 | Pubmed ID: 23728747 Optimization of Malaria Detection Based on Third Harmonic Generation Imaging of Hemozoin Analytical and Bioanalytical Chemistry. Jun, 2013 | Pubmed ID: 23649925 Microstructural Characterization of Vocal Folds Toward a Strain-energy Model of Collagen Remodeling Acta Biomaterialia. Aug, 2013 | Pubmed ID: 23643604 Quantification of Receptor Tyrosine Kinase Activation and Transactivation by G-protein-coupled Receptors Using Spatial Intensity Distribution Analysis (SpIDA) Methods in Enzymology. 2013 | Pubmed ID: 23374183 Image Correlation Spectroscopy: Mapping Correlations in Space, Time, and Reciprocal Space Methods in Enzymology. 2013 | Pubmed ID: 23276542 STICCS Reveals Matrix-dependent Adhesion Slipping and Gripping in Migrating Cells Biophysical Journal. Oct, 2012 | Pubmed ID: 23083710 The Integrin-ligand Interaction Regulates Adhesion and Migration Through a Molecular Clutch PloS One. 2012 | Pubmed ID: 22792239 Determination of Membrane Protein Transporter Oligomerization in Native Tissue Using Spatial Fluorescence Intensity Fluctuation Analysis PloS One. 2012 | Pubmed ID: 22558387 Nonlinear Laser Scanning Microscopy of Human Vocal Folds The Laryngoscope. Feb, 2012 | Pubmed ID: 22252839 Ligand-induced Clustering of EGF Receptors: a Quantitative Study by Fluorescence Image Moment Analysis Biophysical Chemistry. Feb, 2012 | Pubmed ID: 22178063 Quantum Dot Fluorescence Characterizes the Nanoscale Organization of T Cell Receptors for Antigen Biophysical Journal. Dec, 2011 | Pubmed ID: 22261075 Probing the "dark" Fraction of Core-shell Quantum Dots by Ensemble and Single Particle PH-dependent Spectroscopy ACS Nano. Nov, 2011 | Pubmed ID: 22023370 Enhanced Ca2+ Entry Due to Orai1 Plasma Membrane Insertion Increases IL-8 Secretion by Cystic Fibrosis Airways FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Dec, 2011 | Pubmed ID: 21873556 Morphological and Functional Characterization of Cholinergic Interneurons in the Dorsal Horn of the Mouse Spinal Cord The Journal of Comparative Neurology. Nov, 2011 | Pubmed ID: 21618225 Quantification of Receptor Tyrosine Kinase Transactivation Through Direct Dimerization and Surface Density Measurements in Single Cells Proceedings of the National Academy of Sciences of the United States of America. Apr, 2011 | Pubmed ID: 21482778 Revealing Protein Oligomerization and Densities in Situ Using Spatial Intensity Distribution Analysis Proceedings of the National Academy of Sciences of the United States of America. Apr, 2011 | Pubmed ID: 21482753 Live-cell Visualization of Dynamics of HIV Budding Site Interactions with an ESCRT Component Nature Cell Biology. Apr, 2011 | Pubmed ID: 21394086 Fluorescence Microscopy Investigations of Ligand Propagation and Accessibility Under Adherent Cells Biointerphases. Dec, 2010 | Pubmed ID: 21219035 CaMKII Triggers the Diffusional Trapping of Surface AMPARs Through Phosphorylation of Stargazin Neuron. Jul, 2010 | Pubmed ID: 20670832 Second Harmonic Generation Microscopy to Investigate Collagen Configuration: a Pericarditis Case Study Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology. Jul-Aug, 2010 | Pubmed ID: 19632137 A Common Mechanism Underlies the Dark Fraction Formation and Fluorescence Blinking of Quantum Dots ACS Nano. May, 2009 | Pubmed ID: 19385605 Beyond Photobleaching, Laser Illumination Unbinds Fluorescent Proteins The Journal of Physical Chemistry. B. Apr, 2009 | Pubmed ID: 19309095 Stoichiometry of Molecular Complexes at Adhesions in Living Cells Proceedings of the National Academy of Sciences of the United States of America. Feb, 2009 | Pubmed ID: 19168634 Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method Biophysical Journal. Jan, 2009 | Pubmed ID: 19167315 Patterning Protein Concentration Using Laser-assisted Adsorption by Photobleaching, LAPAP Lab on a Chip. Dec, 2008 | Pubmed ID: 19023482 A Guide to Accurate Measurement of Diffusion Using Fluorescence Correlation Techniques with Blinking Quantum Dot Nanoparticle Labels The Journal of Chemical Physics. Jun, 2008 | Pubmed ID: 18554062 Innate Immune-mediated Neuronal Injury Consequent to Loss of Astrocytes Journal of Neuropathology and Experimental Neurology. Jun, 2008 | Pubmed ID: 18520777 Magnitude and Direction of Vesicle Dynamics in Growing Pollen Tubes Using Spatiotemporal Image Correlation Spectroscopy and Fluorescence Recovery After Photobleaching Plant Physiology. Aug, 2008 | Pubmed ID: 18508956 Accurate Measurements of Protein Interactions in Cells Via Improved Spatial Image Cross-correlation Spectroscopy Molecular BioSystems. Jun, 2008 | Pubmed ID: 18493666 Semi-automated Quantification of Filopodial Dynamics Journal of Neuroscience Methods. Jun, 2008 | Pubmed ID: 18394712 Sensitive Detection of Malaria Infection by Third Harmonic Generation Imaging Biophysical Journal. Feb, 2008 | Pubmed ID: 18065455 Fabrication of Protein Gradients for Cell Culture Using a Miniature Squeegee Journal of Biochemical and Biophysical Methods. Apr, 2008 | Pubmed ID: 17597222 Probing Synaptic Signaling with Quantum Dots HFSP Journal. May, 2007 | Pubmed ID: 19404455 Fluorescence Intensity and Intermittency As Tools for Following Dopamine Bioconjugate Processing in Living Cells Journal of Biomedicine & Biotechnology. 2007 | Pubmed ID: 18401443 Advances in Image Correlation Spectroscopy: Measuring Number Densities, Aggregation States, and Dynamics of Fluorescently Labeled Macromolecules in Cells Cell Biochemistry and Biophysics. 2007 | Pubmed ID: 17952641 Actin-myosin Network Reorganization Breaks Symmetry at the Cell Rear to Spontaneously Initiate Polarized Cell Motility The Journal of Cell Biology. Sep, 2007 | Pubmed ID: 17893245 Detection and Correction of Blinking Bias in Image Correlation Transport Measurements of Quantum Dot Tagged Macromolecules Biophysical Journal. Aug, 2007 | Pubmed ID: 17526586 Src-dependent Phosphorylation of Beta2-adaptin Dissociates the Beta-arrestin-AP-2 Complex Journal of Cell Science. May, 2007 | Pubmed ID: 17456551 Investigating Membrane Protein Dynamics in Living Cells Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. Dec, 2006 | Pubmed ID: 17215870 Probing the Integrin-actin Linkage Using High-resolution Protein Velocity Mapping Journal of Cell Science. Dec, 2006 | Pubmed ID: 17158922 A Guide to Accurate Fluorescence Microscopy Colocalization Measurements Biophysical Journal. Dec, 2006 | Pubmed ID: 17012312 Measurement of Monomer-oligomer Distributions Via Fluorescence Moment Image Analysis Biophysical Journal. Nov, 2006 | Pubmed ID: 16935950 K-Space Image Correlation Spectroscopy: a Method for Accurate Transport Measurements Independent of Fluorophore Photophysics Biophysical Journal. Oct, 2006 | Pubmed ID: 16861272 Membrane Lateral Diffusion and Capture of CFTR Within Transient Confinement Zones Biophysical Journal. Aug, 2006 | Pubmed ID: 16714353 Sampling Effects, Noise, and Photobleaching in Temporal Image Correlation Spectroscopy Biophysical Journal. Jan, 2006 | Pubmed ID: 16258048 Two-photon Fluorescent Microlithography for Live-cell Imaging Microscopy Research and Technique. Dec, 2005 | Pubmed ID: 16315236 Accuracy and Dynamic Range of Spatial Image Correlation and Cross-correlation Spectroscopy Biophysical Journal. Aug, 2005 | Pubmed ID: 15923223 Measuring Fast Dynamics in Solutions and Cells with a Laser Scanning Microscope Biophysical Journal. Aug, 2005 | Pubmed ID: 15908582 Fluctuation Correlation Spectroscopy with a Laser-scanning Microscope: Exploiting the Hidden Time Structure Biophysical Journal. May, 2005 | Pubmed ID: 15792971 Spatiotemporal Image Correlation Spectroscopy (STICS) Theory, Verification, and Application to Protein Velocity Mapping in Living CHO Cells Biophysical Journal. May, 2005 | Pubmed ID: 15722439 Spatial Mapping of Integrin Interactions and Dynamics During Cell Migration by Image Correlation Microscopy Journal of Cell Science. Nov, 2004 | Pubmed ID: 15479718 Isolation of Bright Aggregate Fluctuations in a Multipopulation Image Correlation Spectroscopy System Using Intensity Subtraction Biophysical Journal. Jun, 2003 | Pubmed ID: 12770905 Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data George Ashdown1, Elvis Pandžić3, Andrew Cope2, Paul Wiseman4, Dylan Owen1 1Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, 2Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease, King's College London, 3ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine, University of New South Wales Australia, 4Departments of Chemistry and Physic, McGill University JoVE 53749 Immunology and Infection
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data George Ashdown1, Elvis Pandžić3, Andrew Cope2, Paul Wiseman4, Dylan Owen1 1Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, 2Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease, King's College London, 3ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine, University of New South Wales Australia, 4Departments of Chemistry and Physic, McGill University JoVE 53749 Immunology and Infection
