Other Publications (1)
Articles by Taisuke Yoneda in JoVE
Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex Taisuke Yoneda1, Seiichiro Sakai1,2, Hisato Maruoka1, Toshihiko Hosoya1 1RIKEN Brain Science Institute, 2Tokyo Metropolitan Institute of Medical Science Here we describe a procedure for tissue clearing, fluorescent labeling, and large-scale imaging of mouse brain tissue which, thereby, enables visualization of the three-dimensional organization of cell types in the neocortex.
Other articles by Taisuke Yoneda on PubMed
Lattice System of Functionally Distinct Cell Types in the Neocortex Science (New York, N.Y.). 11, 2017 | Pubmed ID: 29097542 The mammalian neocortex contains many cell types, but whether they organize into repeated structures has been unclear. We discovered that major cell types in neocortical layer 5 form a lattice structure in many brain areas. Large-scale three-dimensional imaging revealed that distinct types of excitatory and inhibitory neurons form cell type-specific radial clusters termed microcolumns. Thousands of microcolumns, in turn, are patterned into a hexagonal mosaic tessellating diverse regions of the neocortex. Microcolumn neurons demonstrate synchronized in vivo activity and visual responses with similar orientation preference and ocular dominance. In early postnatal development, microcolumns are coupled by cell type-specific gap junctions and later serve as hubs for convergent synaptic inputs. Thus, layer 5 neurons organize into a brainwide modular system, providing a template for cortical processing.