In JoVE (1)
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Articles by Theo Leitner in JoVE
Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5 Eric Ehrke-Schulz1, Wenli Zhang1, Maren Schiwon1, Thorsten Bergmann1, Manish Solanki1, Jing Liu1, Philip Boehme1, Theo Leitner1, Anja Ehrhardt1 1Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, University Witten/Herdecke A protocol for generation of high-capacity adenoviral vectors lacking all viral coding sequences is presented. Cloning of transgenes contained in the vector genome is based on homing endonucleases. Virus amplification in producer cells grown as adherent cells and in suspension relies on a helper virus providing viral genes in trans.