Articles by Xiaofeng Zhou in JoVE
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis Giovanni Lugli1, Yachana Kataria1, Zachary Richards1, Peter Gann1, Xiaofeng Zhou2, Larisa Nonn1 1Department of Pathology, University of Illinois at Chicago, 2Department of Periodontics, University of Illinois at Chicago The goal of this protocol is to use laser-capture micro-dissection as an effective method to isolate pure populations of cell types from heterogeneous prostate tissues for downstream RNA analysis.
Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs) Madeline Williams*1, Smrithi Prem*1, Xiaofeng Zhou1, Paul Matteson2, Percy Luk Yeung3, Chi-Wei Lu3, Zhiping Pang4, Linda Brzustowicz5, James H. Millonig2, Emanuel Dicicco-Bloom1 1Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services, Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 5Department of Genetics, Rutgers University Neurodevelopmental processes such as proliferation, migration, and neurite outgrowth are often perturbed in neuropsychiatric diseases. Thus, we present protocols to rapidly and reproducibly assess these neurodevelopmental processes in human iPSC-derived NPCs. These protocols also allow the assessment of the effects of relevant growth factors and therapeutics on NPC development.