In JoVE (1)
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Articles by Xiaowei Wang in JoVE
Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy Jingwen Yang1, Yunjun Yan1, Xiaowei Xiang1, Yuchao Xu1, Naiming Zhou2, Tianming Wang1 1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University, 2Institute of Biochemistry, College of Life Sciences, Zhejiang University This protocol describes confocal microscopy detection of G protein-coupled receptor (GPCR) internalization in mammalian cells. It includes the basic cell culture, transfection, and confocal microscopy procedure and provides an efficient and easily interpretable method to detect the subcellular localization and internalization of fusion-expressed GPCR.