Articles by van der Zwaag Marianne in JoVE
Isolation of Mouse Salivary Gland Stem Cells Sarah Pringle1,2, Lalitha S. Y. Nanduri1, van der Zwaag Marianne1, van Os Ronald1,2, Rob P. Coppes1,2 1Department of Cell Biology, University Medical Center Groningen, University of Groningen, 2Department of Radiation Oncology, University Medical Center Groningen, University of Groningen An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.
Other articles by van der Zwaag Marianne on PubMed
Isolation and Characterization of Human Salivary Gland Cells for Stem Cell Transplantation to Reduce Radiation-induced Hyposalivation Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology. Sep, 2009 | Pubmed ID: 19625095 Recently, we showed that transplantation of 100-300 c-Kit(+) stem cells isolated from cultured salispheres ameliorates radiation-damage in murine salivary glands. The aim of this study is to optimize and translate these findings from mice to man.
Regeneration of Irradiated Salivary Glands with Stem Cell Marker Expressing Cells Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology. Jun, 2011 | Pubmed ID: 21719134 Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.