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Epithelial Effects of Intestinal Inflammation on Established Murine Colonoids

June 2nd, 2023
*1, *2,3, 2,3, 2,3
1Division of Pediatric Gastroenterology, Hepatology and Nutrition, UPMC Children’s Hospital of Pittsburgh, 2Division of Pediatric Surgery, UPMC Children’s Hospital of Pittsburgh, 3Department of Surgery, University of Pittsburgh School of Medicine
* These authors contributed equally

Summary
Automatic Translation

Vi beskriver en protokol, der beskriver isoleringen af murinkolonkrypter til udvikling af 3-dimensionelle kolonoider. De etablerede kolonoider kan derefter differentieres terminalt for at afspejle den cellulære sammensætning af værtsepitelet, inden de modtager en inflammatorisk udfordring eller bliver instrueret til at etablere et epitelmonolag.

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