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Investigating Islet Abnormalities and Function with a Pseudoislet Protocol (Video) | JoVE
同步评估人类假胰岛中荧光生物传感器动力学和激素谱
该协议描述了一种使用环磷酸腺苷(cAMP)生物传感器,cAMP原 位 差异检测器(cADDis)和微灌注系统的腺病毒递送,通过原代人假胰岛同步获取和共同注册细胞内信号转导事件以及胰岛素和胰高血糖素分泌的方法。
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