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Isolating Small Extracellular Vesicles and Extracting Their Peptidome

June 30th, 2023
1,2, 1,2, 2,3, 2,3, 2, 2, 2, 1,2
1School of Basic Medical Sciences, Anhui Medical University, 2State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, 3School of Life Sciences, Hebei University

Summary
Automatic Translation

Ce protocole décrit une procédure permettant d’isoler de petites vésicules extracellulaires des macrophages par ultracentrifugation différentielle et d’extraire le peptidome pour l’identification par spectrométrie de masse.

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