Isolating Small Extracellular Vesicles and Extracting Their Peptidome

June 30th, 2023

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מיצוי פפטידים משלפוחיות חוץ-תאיות קטנות מבודדות (sEVs)

03:04 min

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Jiale Cheng^1,2, Jiong Zhu^1,2, Yujiao Liu^2,3, Chen Yang^2,3, Yuehui Zhang², Yuchen Liu², Chaozhi Jin², Jian Wang^1,2

¹School of Basic Medical Sciences, Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, ³School of Life Sciences, Hebei University

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פרוטוקול זה מתאר הליך לבידוד שלפוחיות חוץ-תאיות קטנות ממקרופאגים על ידי אולטרה-צנטריפוגה דיפרנציאלית וחילוץ הפפטידום לזיהוי באמצעות ספקטרומטריית מסות.

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Isolating Small Extracellular Vesicles and Extracting Their Peptidome

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