MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

September 22nd, 2023

Deciphering the Mysteries of Skeletal Muscle Fiber Types Using the MyDoBID Technique (Video) | JoVE

02:11 min

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Isolation of Single-Fiber from Freeze-Dried Human Skeletal Muscle (Video) | JoVE

01:15 min

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MHCI and MHCII Analysis of Single Fibers Isolated from Freeze-Dried Muscle Samples Using Dot Blot (Video) | JoVE

04:08 min

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Heidy K. Latchman*¹, Stefan G. Wette*¹, Dion J. Ellul¹, Robyn M. Murphy¹, Noni T. Frankenberg¹

¹Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment, La Trobe University

* These authors contributed equally

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Este protocolo demuestra el aislamiento de una sola fibra del músculo esquelético humano liofilizado y la clasificación del tipo de fibra de acuerdo con la isoforma de la cadena pesada de miosina (MHC) utilizando la técnica de transferencia de puntos. Las muestras de fibra MHC I y II identificadas se pueden analizar más a fondo para detectar diferencias específicas del tipo de fibra en la expresión de proteínas mediante Western blot.

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MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

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