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MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

September 22nd, 2023
*1, *1, 1, 1, 1
1Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment, La Trobe University
* These authors contributed equally

Summary
Automatic Translation

Denne protokol demonstrerer enkeltfiberisolering fra frysetørret human skeletmuskulatur og fibertypeklassificering i henhold til Myosin heavy chain (MHC) isoform ved hjælp af dot blotting-teknikken. Identificerede MHC I og II fiberprøver kan derefter analyseres yderligere for fibertypespecifikke forskelle i proteinekspression ved hjælp af western blotting.

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