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MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

September 22nd, 2023
*1, *1, 1, 1, 1
1Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment, La Trobe University
* These authors contributed equally

Summary
Automatic Translation

Detta protokoll demonstrerar isolering av enstaka fibrer från frystorkad mänsklig skelettmuskulatur och fibertypsklassificering enligt isoform av Myosin heavy chain (MHC) med hjälp av dot blotting-tekniken. Identifierade MHC I- och II-fiberprover kan sedan analyseras ytterligare för fibertypsspecifika skillnader i proteinuttryck med hjälp av western blotting.

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