JoVE Biology Newsletter – December 2018

Indrani Mukherjee, Ph.D.
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Sperm Collection of Differential Quality

Dr. Andrew Benson’s lab, at University of Georgia, describes a density gradient centrifugation technique and its application in sperm physiology research.

Mouse Blood-Testis Barrier Integrity

Dr. Ke Zheng’s lab, at Nanjing Medical University, presents a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into mouse testes.

ChIP of Murine Brown Adipose Tissue

Dr. Valentina Perissi’s lab, at Boston University, describes a protocol for efficient chromatin immunoprecipitation followed by high-throughput DNA sequencing of brown adipose tissue.

Protein-Protein Interactions at Cell-Cell Contacts

Dr. Salvatore Chiantia’s lab, at University of Potsdam, describes a fluorescence fluctuation spectroscopy-based approach to investigate interactions among proteins mediating cell-cell interactions.

Abnormalities on Fibrin Clot Structure

Dr. Hyung Jin Ahn’s lab, at Rockefeller University, presents two methods that can be used individually or in combination to analyze the effect of beta-amyloid on fibrin clot structure.

RNA-based Reprogramming of Human Fibroblasts into iPSCs

Dr. Ganna Bilousova’s lab, at University of Colorado, describes a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells.

Prostate Organoid Research Techniques and Methods: Three-Dimensional (3D) Culture, Spin-down, Dimpled Parafilm and Fusion Method

Techniques for high throughput lifespan measurement in invertebrate model systems

RNA/DNA In Situ Hybridization techniques

Comprehensive Guide to Inflammation Detection Methodologies

Genetics

This Sci Ed collection focuses on genetics and incorporates five broad subdisciplines.

Cell Biology

This Sci Ed collection provides a glimpse into the field of cell biology and profiles five important cellular phenomena.

Basic Methods in Cellular and Molecular Biology

This Sci Ed collection demonstrates how to execute basic techniques commonly used in cellular and molecular biology.

General Laboratory Techniques

This Sci Ed collection is a comprehensive video guide for appropriate lab animal care and use.

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