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Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants
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Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants

Article DOI: 10.3791/2082-v • 07:00 min • December 1st, 2010
December 1st, 2010 •
1, 2, 2
1 Neuroscience Lab/ Fac. Psicologia, University of Colima, 2Department of Neurosurgery, Johns Hopkins University

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In this article, we show a method to make glass capillary needles with a 50-μm lumen. This technique significantly reduces the brain damage, minimizes passive diffusion of drugs and allows a precise targeting into the rodent brain.

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Deep Brain Structures Microinjections Drug Delivery Viral Vectors Cell Transplants Brain Parenchyma Injecting Needle Brain Lesion Mouse Brain Glass Capillary Needles Brain Damage Precise Targeting Rodent Brain Small Volumes Bleeding Risks Passive Diffusion Injection Techniques Collateral Damage
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