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Biology
一个渐变产生的微流体装置,细胞生物学
一个渐变产生的微流体装置,细胞生物学
JoVE Journal
Biology
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JoVE Journal Biology
A Gradient-generating Microfluidic Device for Cell Biology

一个渐变产生的微流体装置,细胞生物学

Full Text
15,933 Views
11:05 min
August 30, 2007

DOI: 10.3791/271-v

Bong Geun Chung1, Amir Manbachi1, Wajeeh Saadi1, Francis Lin1, Noo Li Jeon1, Ali Khademhosseini1

1Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine,Brigham and Women's Hospital

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Please note that some of the translations on this page are AI generated. Click here for the English version.

我们描述了一个协议梯度产生的微流体装置,可以产生良好定义的微环境中的空间和时间的梯度微细。在这种方法中,梯度产生的微流体装置可用于研究细胞定向迁移,胚胎发育,伤口愈合,癌症转移。

我叫江。我是哈佛大学和麻省理工学院健康、科学和技术的海报研究员。我叫 Amir Manchi,是麻省理工学院哈佛大学健康科学与技术系 SANE 实验室的本科生教授。

这张幻灯片展示了如何制作设备的方案过程,因此我只需在硅蒸汽上涂上 SUA 50 旋涂,然后在硅蒸汽上进一步放置掩模,然后进行 UUV 曝光和显影。我们终于可以得到,把这些垫子放在硅胶蒸气上。之后,我们只需铸造 PDMS,然后爬行和烘烤,然后进行小冲头,然后我们就可以使用氧气等离子体在 PDMS 设备和直的 glace 之间粘合。

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第7期 细胞生物学 组织工程 微流体 细胞迁移 梯度

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