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Journal / Bioengineering / Lignin Herunterregulierung
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JoVE Journal Bioengineering
Lignin Herunterregulierung
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Lignin Herunterregulierung

Article DOI: 10.3791/51340-v • 14:43 min • July 23rd, 2014
July 23rd, 2014 •
1, 2, 3, 4
1The School of Plant Sciences, University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, 3The Institute for Sustainable and Renewable Resources, The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences, Michigan State University

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Ein Doppelstrang-RNA-Interferenz (dsRNAi)-Technik wird eingesetzt, um nach unten zu regulieren den Mais Cinnamoyl Coenzym A-Reduktase (ZmCCR1)-Gen zu niedrigeren Pflanzen Ligningehalt. Lignin Down-Regulation aus der Zellwand wird durch mikroskopische Analysen visualisiert und durch die Klason Verfahren quantifiziert. Änderungen der Zusammensetzung in kristalliner Cellulose und Hemicellulose werden analysiert.

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Bioengineering , dsRNAi Klason Lignin Messung Zellwandkohlenhydratanalyse Gaschromatographie (GC)
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