Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
 

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Article doi: 10.3791/1229
March 25th, 2009

Summary March 25th, 2009

Whole mount in situ hybridization is one of the most widely used techniques in developmental biology. Here, we present a high-resolution double fluorescent in situ hybridization protocol for analyzing the precise expression pattern of a single gene and for determining the overlap of the expression domains of two genes. We include a propidium iodide nuclear counter-stain to highlight tissue organization.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter