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March 25, 2009
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Summary
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Whole mount in situ hybridization is one of the most widely used techniques in developmental biology. Here, we present a high-resolution double fluorescent in situ hybridization protocol for analyzing the precise expression pattern of a single gene and for determining the overlap of the expression domains of two genes. We include a propidium iodide nuclear counter-stain to highlight tissue organization.
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Cite this Article
Brend, T., Holley, S. A. Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization. J. Vis. Exp. (25), e1229, doi:10.3791/1229 (2009).
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