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平山准备观察斑马鱼胚胎标本染色，并通过分析全山<em>原位</em>杂交

JoVE Journal
Biology

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JoVE Journal Biology

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

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平山准备观察斑马鱼胚胎标本染色，并通过分析全山<em>原位</em>杂交

DOI:

10.3791/51604-v

•

06:36 min

•

July 17, 2014

•

Christina N. Cheng, Yue Li, Amanda N. Marra, Valerie Verdun, Rebecca A. Wingert

¹Department of Biological Sciences,University of Notre Dame

Chapters

00:05Title
01:37Dissection and Initial Deyolking of Embryo
02:26Fine Deyolking of the Embryo
03:18Mounting Embryo on a Glass Slide
04:23Results: In situ Hybridization of Flat-mounted Embryos
05:58Conclusion

Summary

Automatic Translation

斑马鱼的胚胎是发育生物学研究的一个很好的模型。胚胎发育过程中，斑马鱼发展与蛋黄质量，其中介绍的样品观察和分析，三维的挑战。本协议描述了如何创建原位（WISH）染色斑马鱼胚胎标本整装二维平板安装的准备工作。

Tags

Keywords: Zebrafish Embryo Developmental Stages Whole Mount In Situ Hybridization (WISH)Flat Mount Preparation Gene Expression Embryonic Structures Developmental Biology Biomedical Research

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