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JoVE Journal
Immunology and Infection

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荧光标记基因的基因枪转化成机会性真菌病原
 
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荧光标记基因的基因枪转化成机会性真菌病原

Article DOI: 10.3791/52666-v 07:32 min March 19th, 2015
March 19th, 2015

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总结概括

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生物射弹转化是用于产生的DNA的稳定整合入条件致病菌新生隐球菌通过同源重组的基因组中的方法。我们将展示一个结构,它具有融合到荧光标记mCherry到新生隐球菌基因编码醋酸激酶的基因枪转化。

Tags

分子生物学,第97,基因破坏,
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