Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Dasol Lee; Minkyung Kim; Sunae So; Inki Kim; Gwanho Yoon; Kyunghoon Kim; Junsuk Rho

doi:10.3791/55968

JoVE Journal
Engineering

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JoVE Journal Engineering

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Hyperlens 综合显微镜和超分辨率成像的演示

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10:01 min

September 8, 2017

DOI: 10.3791/55968-v

Dasol Lee*¹, Minkyung Kim*¹, Sunae So*¹, Inki Kim¹, Gwanho Yoon¹, Kyunghoon Kim², Junsuk Rho^1,3

¹Department of Mechanical Engineering,Pohang University of Science and Technology (POSTECH), ²School of Mechanical Engineering,Sungkyunkwan University, ³Department of Chemical Engineering,Pohang University of Science and Technology (POSTECH)

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hyperlens 的应用是一种新型的超分辨率成像技术, 由于其在 real-time 成像中的优越性和常规光学的简单实现。在这里, 我们提出了一个描述球面 hyperlens 的制作和成像应用的协议。

该实验程序的总体目标是演示二维超透镜器件的制造过程和亚衍射成像。这种新颖的超分辨率成像技术具有实时成像和与传统光学器件的简单实现等优点。该方法可以帮助回答超分辨率成像领域的关键问题，例如对低于馏分极限的活细胞和动态纳米粒子进行成像。

HyperLens 是一种具有多层结构的特殊球面透镜，具有平坦的双曲线色散，支持高频信息的放大和类似光学元件的实时分辨率。球形超透镜的主要优点是它可以在可见频率下放大二维信息。球面超透镜也可以很容易地集成到传统显微镜中，而无需额外的复杂系统。

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工程问题 127 超分辨率远场成像衍射极限双曲超常材料 hyperlens real-time 成像新颖显微镜纳米