Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-<em>O</em>-thiophenylmethyl Modification and Characterization via Circular Dichroism

Andrew J. Francis; Marino J. E. Resendiz

doi:10.3791/56189

Journal

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化学

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用于固相合成含有2'-<em> 0</em通过圆二色性修饰和表征

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-<em>O</em>-thiophenylmethyl Modification and Characterization via Circular Dichroism

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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

用于固相合成含有2'-<em> 0</em通过圆二色性修饰和表征

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18,883 Views

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11:37 min

•

July 28, 2017

DOI:

10.3791/56189-v

DOI:

10.3791/56189-v

•

11:37 min

July 28, 2017

•

6 Views

Andrew J. Francis¹, Marino J. E. Resendiz¹

¹Department of Chemistry,University of Colorado Denver

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Summary

本文提供了在C2'- O位上修饰的RNA的十二聚体的固相合成，纯化和表征的详细程序。 UV-vis和圆二色性光度分析用于量化和表征结构方面，即单链或双链。

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用于固相合成含有2'-<em> 0</em通过圆二色性修饰和表征

成績單

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Summary

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