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Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors

Article DOI: 10.3791/59101-v • 05:19 min • March 29th, 2019
March 29th, 2019 •
1, 2, 3, 3
1Department of Surgery, Division of Urology, NorthShore University HealthSystem, 2Department of Radiology, Northwestern University Feinberg School of Medicine, 3Department of Urology, Northwestern University Feinberg School of Medicine

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Murine bladder tumors are induced with the N-butyl-N-(4-hydroxybutyl) nitrosamine carcinogen (BBN). Bladder tumor generation is heterogeneous; therefore, an accurate assessment of tumor burden is needed before randomization to experimental treatment. Here we present a fast, reliable MRI protocol to assess tumor size and stage.

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Magnetic Resonance Imaging Carcinogen-induced Murine Bladder Tumors Reproducibly Generate Bladder Tumor Burden MRI Operator Independent Rapid Imaging Approach Short Acquisition Times High Diagnostic Quality High-throughput Assays Tumor Development Induce Bladder Cancer Tumor Formation BBN Carcinogen Opaque Drinking Water Container C57 Black Six Mice Ad Libitum Monitor Animals Sign Of Distress Local IACUC Guidelines Exposure Time One Milliliter Syringe Sterile Saline Subcutaneous Injection Imaging Holder Rectal Temperature Probe Physiological Recording Computer Body Temperature Monitoring 4-channel Quadrature Receiver Coil
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