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JoVE Journal Cancer Research
Evaluering af differentierings kapaciteten af mus prostata Epiteliale celler ved hjælp af Organoid kultur
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Evaluering af differentierings kapaciteten af mus prostata Epiteliale celler ved hjælp af Organoid kultur

Article DOI: 10.3791/60223-v • 10:38 min • November 22nd, 2019
November 22nd, 2019 •
*1, *1, 2, 2, 2,3,4,5,6
1Molecular Biology Interdepartmental Program, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 6Molecular Biology Institute, University of California, Los Angeles
* These authors contributed equally

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Mus prostata organoider repræsenterer en lovende sammenhæng for at evaluere mekanismer, der regulerer differentiering. Dette papir beskriver en forbedret tilgang til at etablere prostata organoider, og introducerer metoder til (1) indsamle protein lysat fra organoider, og (2) Fix og plette organoider for hele Mount confokal mikroskopi.

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Kræftforskning organoid prostata epithelium stamfader basal luminal differentiering mus
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