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Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Article DOI: 10.3791/60272-v • 07:54 min • December 21st, 2019
December 21st, 2019 •
1, 1, 1, 2, 2
1MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), Division of Nuclear Medicine and Molecular Imaging, University Hospitals and University of Geneva, 2Division of Thoracic and Endocrine Surgery, University Hospitals and University of Geneva

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This article describes the generation of an orthotopic mouse model of human pleural mesothelioma by implantation of H2052/484 mesothelioma cells into the pleural cavity of immunocompromised athymic mice. The longitudinal monitoring of the development of intrapleural tumors was assessed by non-invasive multimodal [18F]-2-fluoro-2-deoxy-D-glucose positron emission tomography and computed tomography imaging.

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Implantation Monitoring PET/CT Orthotopic Model Human Pleural Mesothelioma Athymic Mice Development Treatment Diagnosis Pathology Pre-clinical Model Microenvironment Non-invasive Molecular Imaging Longitudinal Monitoring Anesthesia System Surgical Area Laminar Flow Hood Disinfectant Micro-isolated SPF Cages Heating Pad Povidone-iodine Solution Hamilton Syringe Gauze Cotton Swabs Surgery Instruments Micropipettes And Tips Anesthetized Mouse Subcutaneous Injection Buprinorphine Post-operative Pain Relief Skin Incision Surrounding Fat And Muscles Cell Suspension
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