/

免疫与感染

/

粪便（微量）RNA分离

JoVE 杂志
免疫与感染

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志免疫与感染

Fecal (micro) RNA Isolation

粪便（微量）RNA分离

Please note that all translations are automatically generated. Click here for the English version.

4,303 Views

•

05:35 min

•

October 28, 2020

DOI:

10.3791/61908-v

DOI:

10.3791/61908-v

•

05:35 min

October 28, 2020

•

3 Views

Fyonn H. Dhang^1,2, Howard L. Weiner^1,2, Shirong Liu^1,2

¹Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center,Brigham and Women’s Hospital and Harvard Medical School, ²Evergrande Center for Immunologic Diseases,Harvard Medical School and Brigham and Women’s Hospital

成績單

Summary

该协议从动物和人类受试者的粪便样本中分离出高质量的总RNA。商业 miRNA 分离试剂盒具有显著适应性，以优化数量和质量分离纯 RNA。RNA分离株适用于大多数下游RNA检测，如测序、微阵列和RT-PCR。

Explore More Videos

Fecal MicroRNA RNA Isolation DPBS Acid Phenol Chloroform Ethanol Filter Cartridge Wash Solution

Read Article