Journal
/
生物学
/
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

DOI:
10.3791/62008-v

•

09:08 min

•

December 22, 2020

•

, , , , , , , , , ,
1The Graduate Center – Advanced Science Research Center, Neuroscience Initiative,The City University of New York, 2The City College of New York, CUNY, 3The Bronx High School of Science, 4The Graduate Center – Advanced Science Research Center, Structural Biology Initiative,The City University of New York, 5The Graduate Center – Advanced Science Research Center, MALDI MS Imaging Joint Core Facility,The City University of New York

Chapters

  • 00:04Introduction
  • 00:46Tissue Preparation
  • 01:18Cryosection Acquisition
  • 03:24Matrix Preparation
  • 04:13Matrix Deposition
  • 06:16Results: Representative Output with Selected m/z Spectra from Mass Spectrometry Acquired at 50-micron Lateral Resolution
  • 07:37Conclusion

Summary

自动翻译

自动翻译

The goal of this protocol is to provide detailed guidance on the sample preparation when planning for experiments using MALDI MSI to maximize metabolic and molecular detection in biological samples.

Tags

MALDI Imaging Mass SpectrometryMetabolite Abundance And DistributionCryosectioningSlide PreparationMALDI Slide ConductivitySample Drying

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

MALDI Sample Preparation: the Ultra Thin Layer Method

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Sample Preparation for Mass Cytometry Analysis

Cellular Redox Profiling Using High-content Microscopy

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

Read Article