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Isolement des cellules valvulaires interstitielles de souris pour étudier la calcification de la valve aortique in vitro
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Isolement des cellules valvulaires interstitielles de souris pour étudier la calcification de la valve aortique in vitro

Article DOI: 10.3791/62419-v • 05:47 min • May 10th, 2021
May 10th, 2021 •
1, 1,2,3
1Cardiovascular Research Center, The Icahn School of Medicine at Mount Sinai, 2Diabetes, Obesity and Metabolism Institute, Department of Medicine, The Icahn School of Medicine at Mount Sinai, 3Graduate School of Biomedical Sciences, The Icahn School of Medicine at Mount Sinai

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Cet article décrit l’isolement des cellules de valve aortique de souris par un procédé en deux étapes de collagénase. Les cellules valvulaires isolées de la souris sont importantes pour effectuer différents essais, tels que ce test de calcification in vitro, et pour étudier les voies moléculaires menant à la minéralisation de la valve aortique.

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Biologie Numéro 171
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