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Opprettholde humant glioblastom cellulært mangfold ex vivo ved bruk av tredimensjonal organoidkultur

Article DOI: 10.3791/63745-v • 07:11 min • August 25th, 2022
August 25th, 2022 •
1, 2, 1, 2
1Department of Neurological Surgery, Cleveland Clinic, 2Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic

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Her beskriver vi en metode for å generere glioblastom (GBM) organoider fra primære pasientprøver eller pasientavledede cellekulturer og opprettholde dem til modenhet. Disse GBM-organoidene inneholder fenotypisk forskjellige cellepopulasjoner og gjenskaper tumormikromiljøer ex vivo.

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