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Quantification of Immunostained Caspase-9 in Retinal Tissue

Article DOI: 10.3791/64237-v • 05:18 min • July 25th, 2022
July 25th, 2022 •
1, 2, 1, 1, 3, 1, *1, *1,4,5
1Department of Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, 2Department of Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons, Columbia University, 3NCI-designated Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, 4Department of Neurology, Vagelos College of Physicians and Surgeons, Columbia University, 5The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University
* These authors contributed equally

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Presented here is a detailed immunohistochemistry protocol to identify, validate, and target functionally relevant caspases in complex tissues.

Tags

Immunostain Caspase-9 Retinal Tissue Immunohistochemical Method Cell Biology Therapeutic Targets Confocal Microscopy Stained Retinal Sections Fiji Color Channels Z Stack Max Intensity Projection Brightness And Contrast Interface Pixel Value
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