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Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

Article DOI: 10.3791/1105-v • 09:36 min • February 9th, 2009
February 9th, 2009 •
1, 1, 1
1Department of Zoology, University of British Columbia - UBC

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The genome of the influenza A virus consists of eight separate complexes of RNA and proteins, termed viral ribonucleoprotein complexes (vRNPs). This paper describes the glycerol gradient purification and transmission electron microscopy visualization of influenza A vRNPs.

Tags

Purification Visualization Influenza A Viral Ribonucleoprotein Complexes VRNPs Viral Envelope Nuclear Import Replication Isolated VRNPs Procedure Disruption Detergents Glycerol Gradient Velocity Sedimentation SDS-PAGE Coomassie Blue Staining Peak Fractions Concentration Centrifugation Integrity Verification Transmission Electron Microscopy
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