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Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Article DOI: 10.3791/1863-v • 15:03 min • March 24th, 2010
March 24th, 2010 •
1, 1, 1
1Department of Biological Sciences and Purdue Center for Cancer Research, Purdue University

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Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae.

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Morphological Phenotype Protein Concentration Budding Yeast Gene Deletion Protein Overexpression Phenotype Development Population Level Single-cell Level Saccharomyces Cerevisiae Endocytic Adaptor Protein Epsin Dominant Negative Approach ENTH Domain Cell Division Defect Mislocalization Of Scaffolding Proteins Septins
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