JoVE Journal
Biology
Biology
Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.
Kapitel
Summary
We describe a method for the quantitative, real-time measurement of DNA glycosylase and AP endonuclease activities in cell nuclear lysates. The assay yields rates of DNA Repair activity amenable to kinetic analysis and is adaptable for quantification of DNA Repair activity in tissue and tumor lysates or with purified proteins.