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An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Article DOI: 10.3791/4233-v • 10:00 min • August 31st, 2012
August 31st, 2012 •
1,2, 1, 1,3
1Medications Development, Ernest Gallo Clinic and Research Center, University of California, San Francisco, 2Clinical Pharmacology and Experimental Therapeutics, University of California, San Francisco, 3Translational Research Institute and the Institute for Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia

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Summary
Automatic Translation

We developed a software platform that utilizes Imaris Neuroscience, ImarisXT and MATLAB to measure the changes in morphology of an undefined shape taken from three-dimensional confocal fluorescence of single cells. This novel approach can be used to quantify changes in cell shape following receptor activation and therefore represents a possible additional tool for drug discovery.

Tags

Analytical Tool Cellular Morphology Changes Three-dimensional Fluorescence Images Software Analysis Tools Fluorescence Image Measurement 3D Datasets Cellular Dynamics Cellular Plasticity Microscopy Instruments Multispectral Fluorescence Images Confocal Stacks 3D Representation Design-based Stereology Methods Automated Analytic Method Cell Morphology Analysis Protein Localization Analysis Receptor Trafficking Analysis Meta-Morph
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