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Journal / Bioengineering / Quantitative Locomotion Study of Freely Swimming…
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Bioengineering

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JoVE Journal Bioengineering
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Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

Article DOI: 10.3791/4412-v • 10:03 min • October 25th, 2012
October 25th, 2012 •
1, 2, 1
1Physics & Astronomy Department, Vassar College, 2Biology Department, Vassar College

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Summary
Automatic Translation

Microscopic organisms like the free-swimming nematode C. elegans, live and behave in a complex three-dimensional environment. We report on a novel approach that provides analysis of C. elegans using diffraction patterns. This approach consists of tracking the temporal periodicity of diffraction patterns generated by directing laser light through a cuvette.

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Quantitative Locomotion Study Freely Swimming Micro-organisms Laser Diffraction Microscope-based Approaches Real-time Analysis C. Elegans Cuvette Oscillation Frequencies Far-field Diffraction Patterns Waveforms Of Nematodes Fraunhofer Diffraction Pattern Fourier Transform Soil-dwelling Nematodes Crawling Locomotory Pattern Swimming Pattern
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