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Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography

Article DOI: 10.3791/51183-v • 11:18 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 1,4
1Department of Bioengineering, Stanford University, 2Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Umeå University, 3Campus de Cantoblanco, Universidad Autonoma de Madrid, 4Department of Microbiology and Immunology, Stanford University School of Medicine

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Summary
Automatic Translation

The bacterial cell wall is composed of peptidoglycan, a macromolecular network of sugar strands crosslinked by peptides. Ultra Performance Liquid Chromatography provides high resolution and throughput for novel discoveries of peptidoglycan composition. We present a procedure for the isolation of cell walls (sacculi) and their subsequent preparation for analysis via UPLC.

Tags

Isolation Preparation Bacterial Cell Walls Compositional Analysis Ultra Performance Liquid Chromatography Cell Shape Mechanical Integrity Turgor Pressures Peptidoglycan Antibiotic Target Genetic Studies Structural Studies Cell Biological Studies Enzymatic Activities Chemical Composition High Performance Liquid Chromatography HPLC Ultra Performance Liquid Chromatography UPLC
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