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1Murdoch Childrens Research Institute,The Royal Children’s Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences,The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences,Monash University, Clayton
Kapitel
- 00:05Titel
- 01:14Foreskin and Lymphatic Malformation Cell Suspension Preparation
- 04:01Antibody Staining of Lymphatic Endothelial Cell (LEC) Surface Markers
- 05:49Results: Representative Foreskin and Lymphatic Malformation LECs
- 06:53Conclusion
The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies.
