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Biochemistry
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
 

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Article DOI: 10.3791/55779-v 14:55 min September 17th, 2017
September 17th, 2017

Kapitel

Summary

Structures of supramolecular protein assemblies at atomic resolution are of high relevance because of their crucial roles in a variety of biological phenomena. Herein, we present a protocol to perform high-resolution structural studies on insoluble and non-crystalline macromolecular protein assemblies by magic-angle spinning solid-state nuclear magnetic resonance spectroscopy (MAS SSNMR).

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Keywords: Solid-state NMR Macromolecular Assemblies Protein Structure Isotopic Labeling In Vitro Assembly Sample Preparation Structural Characterization Atomic Resolution
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