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Detection of Detergent-sensitive Interactions Between Membrane Proteins
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Biochemie
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JoVE Journal Biochemie
Detection of Detergent-sensitive Interactions Between Membrane Proteins

Detection of Detergent-sensitive Interactions Between Membrane Proteins

DOI:
10.3791/57179-v

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10:09 min

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March 07, 2018

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, ,
1Department of Biochemistry,Boston University School of Medicine

Kapitel

  • 00:04Titel
  • 01:05Handling of the Peptide and Cells
  • 03:02Binding of His-tagged Proteins to the HisPur Cobalt Beads
  • 04:39Binding of the Peptide to the His-tagged Protein Immobilized on the Beads
  • 05:59Elution from Cobalt Beads
  • 07:07Electrophoresis, Western Blotting, and Analysis of Results
  • 07:58Results: Use of Immunoblot Assay to Study the Interaction between Sortilin Immobilized on Cobalt Beads and myc-tagged Peptide
  • 09:18Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe a protocol for detection of detergent-sensitive interactions between membrane proteins using binding of the sorting receptor, sortilin, to the first luminal loop of the glucose transporter protein, GLUT4, as an example.

Tags

Membrane ProteinsDetergent-sensitive InteractionsHistidine-taggedMyc-taggedCo-immunoprecipitationCell Lysate3T3-L1 Pre-adipocytesTransfectionProtein InteractionsBiochemie

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