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Journal / Biochemistry / 형광 기반 측정 인스파디딜세린/포스파디딜리노시톨 4-인산염 교환 막…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
형광 기반 측정 인스파디딜세린/포스파디딜리노시톨 4-인산염 교환 막 간
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Journal (Biochemistry)
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
Journal (Biochemistry)
A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
 
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형광 기반 측정 인스파디딜세린/포스파디딜리노시톨 4-인산염 교환 막 간

Article DOI: 10.3791/62177-v • 08:49 min • March 14th, 2021
March 14th, 2021 •
*1, *1, 1, 1
1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire
* These authors contributed equally

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Summary
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여기서, 우리는 단백질 추출물과 트랜스포파디딜리노시톨 4-인산염이 체외에서인산염을 배출하는지 여부를 결정하기 위해 형광 지질 센서와 리포좀을 사용하는 프로토콜을 설명합니다.

Tags

생화학 제169 지질 전달 단백질 OSBP 관련 단백질 인산절실세린 인산염 4-인산염 형광 재조합 단백질 리포솜
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