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Biochemistry
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형광 기반 측정 인스파디딜세린/포스파디딜리노시톨 4-인산염 교환 막 간
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Biochemistry
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JoVE Journal
Biochemistry
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Please note that all translations are automatically generated.
Click here for the English version.
형광 기반 측정 인스파디딜세린/포스파디딜리노시톨 4-인산염 교환 막 간
DOI:
10.3791/62177-v
•
08:49 min
•
March 14, 2021
•
Souade Ikhlef*
1
,
Nicolas-Frédéric Lipp*
1
,
Maud Magdeleine
,
Guillaume Drin
1
Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire
Chapters
00:04
Introduction
00:59
Measurement of PS or PI(4)P Extraction
02:44
Real-time Measurement of PS Transport
04:54
Real-time Measurement of PI(4)P Transport
06:23
Results: Membrane-Exchange of Phosphatidylserine/ Phosphatidylinositol 4-Phosphate Using Fluorescent Sensors
07:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
여기서, 우리는 단백질 추출물과 트랜스포파디딜리노시톨 4-인산염이
체외에서
인산염을 배출하는지 여부를 결정하기 위해 형광 지질 센서와 리포좀을 사용하는 프로토콜을 설명합니다.
Tags
Fluorescence
Phosphatidylserine
Phosphatidylinositol 4-phosphate
Lipid Transfer
Membrane
Liposomes
NBD-C2 Lact
Lipid Sensor
Lipid Transfer Protein
NBD-PH FAPP Probe
PI(4)P Extraction Assay
HEPES Potassium Acetate Buffer
Magnesium Chloride
Phosphatidylcholine
Phosphatidylethanolamine
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