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Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Article DOI: 10.3791/51543-v • 11:58 min • June 25th, 2014
June 25th, 2014 •
1, 1, 1, 1, 1
1Department of Translational Oncology, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ)

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Linear-amplification mediated (LAM)-PCR is a method developed to identify the exact positions of integrating viral vectors in the genome. The technique has evolved to be the superior method to study clonal dynamics in gene therapy patients, biosafety of novel vector technologies, T-cell diversity, cancer stem cell models, etc.

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Linear Amplification Mediated PCR LAM-PCR Gene Therapy Integrating Vector Systems Retroviral Vectors Clonal Fate Proto-oncogene High Sensitivity High Specificity Preamplification Step Biotinylated Vector Specific Primers Solid Phase Magnetic Beads Unknown DNA Known DNA Restriction Digest Integration Sites Comprehensive Analysis Lentiviral Vector
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