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Immunology and Infection

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JoVE Journal Immunology and Infection
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A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

Article DOI: 10.3791/54255-v • 06:28 min • August 12th, 2016
August 12th, 2016 •
1,2,3, 4, 4, 5, 1,2,6
1Centre for Biomedical Research, Macfarlane Burnet Institute for Medical Research and Public Health, 2Department of Infectious Diseases, Monash University, 3Department of Microbiology and Immunology, University of Melbourne, 4Department of Microbiology, The University of the West Indies, 5Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, 6Department of Medicine, Monash University

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Resumen
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Monocytes are integral components of the human innate immune system that rely on glycolytic metabolism when activated. We describe a flow cytometry protocol to measure glucose transporter expression and glucose uptake by total monocytes and monocyte subpopulations in fresh whole blood.

Tags

Flow Cytometry Glucose Uptake Glucose Transporter Expression Monocyte Subpopulations Whole Blood Immunology Field Immune Cell Metabolism Disease Outcomes Quick Method Easy To Perform Small Volume Of Blood Anticoagulant Tubes Lysing Solution Wash Buffer Supernatants Removal Specific Monocyte Subpopulations Identification Antibody Staining Incubation Wash Solution Fixative Solution
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