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Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants
JoVE Journal
Biology
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JoVE Journal Biology
Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants
DOI:

10:39 min

June 01, 2009

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Chapters

  • 00:05Title
  • 01:05Introduction
  • 01:40Preparing the Embryos
  • 03:23Preparation of Dissection Needles
  • 04:48Transplantation Procedure
  • 09:34Interpreting the Experimental Outcome
  • 10:01Conclusion

Summary

Automatic Translation

Lens development involves interactions with other tissues. Several zebrafish eye mutants are characterized by an abnormally small lens size. Here we demonstrate a lens transplantation experiment to determine whether this phenotype is due to intrinsic causes or defective interactions with tissues that surround the lens.

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